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Shedding of N-acetylglucosaminyltransferase-V is regulated by ... - Nature.com

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Abstract The number of N -glycan branches on glycoproteins is closely related to the development and aggravation of various diseases. Dysregulated formation of the branch produced by N -acetylglucosaminyltransferase-V (GnT-V, also called as MGAT5) promotes cancer growth and malignancy. However, it is largely unknown how the activity of GnT-V in cells is regulated. Here, we discover that the activity of GnT-V in cells is selectively upregulated by changing cellular N -glycans from mature to immature forms. Our glycomic analysis further shows that loss of terminal modifications of N -glycans resulted in an increase in the amount of the GnT-V-produced branch. Mechanistically, shedding (cleavage and extracellular secretion) of GnT-V mediated by signal peptide peptidase-like 3 (SPPL3) protease is greatly inhibited by blocking maturation of cellular N -glycans, resulting in an increased level of GnT-V protein in cells. Alteration of cellular N -glycans hardly impairs expression or localizati...
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MLMs like Young Living Essential Oils and Doterra are using coronavirus to recruit sellers - Vox.com

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"If you want to keep away from working around crowds of people and you're looking for a way to earn money from home ~~ I've got an excellent opportunity for you!...Avoid exposure to the Corona Virus and still provide for your family in such an amazing way." So begins a comment posted to a Young Living Essential Oils group page on Facebook. The post went up on March 7, with the offer of "working away from crowds" coming in response to the spread of Covid-19, which in the weeks following has become a global pandemic, causing social distancing to become the de-facto law of the land, and rightfully so. What this poster, and others like her, are presenting is an opportunity to start a business that doesn't require any social contact at all. That business model is known as multilevel marketing, or MLM. Young Living Essential Oils is one of many MLMs, also known as network-marketing companies. Some of these businesses sell leggings, others diet shakes, othe...
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Flanked by a Ferrari, a Maserati, a Bentley, a Rolls-Royce and a Lamborghini, Dallin Larsen paced the stage, swigging deeply from a bottle in his hand. "I'll tell you what," said the tanned 49-year-old, opening his arms to the 4,000-person crowd, people are "looking for something they can count on, they can depend on, that's constant." The stirring scene would not be out of place at a megachurch revival—except Larsen's event, organized this June in Orlando, Fla., was bent on earning sales rather than salvation. The object of hope was not God but a dark purple fruit juice called MonaVie. The rich syrupy blend of Brazilian açai (pronounced "ah-sigh-ee") berries and 18 other fruits has gained a cult following among those who say it can kill pain, disease and malnutrition. Packaged in wine bottles like the one Larsen gripped onstage, MonaVie retails for around $40 a pop and isn't available in stores. Instead, the Utah-based company tore a pag...
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