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Shedding of N-acetylglucosaminyltransferase-V is regulated by ... - Nature.com

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Abstract The number of N -glycan branches on glycoproteins is closely related to the development and aggravation of various diseases. Dysregulated formation of the branch produced by N -acetylglucosaminyltransferase-V (GnT-V, also called as MGAT5) promotes cancer growth and malignancy. However, it is largely unknown how the activity of GnT-V in cells is regulated. Here, we discover that the activity of GnT-V in cells is selectively upregulated by changing cellular N -glycans from mature to immature forms. Our glycomic analysis further shows that loss of terminal modifications of N -glycans resulted in an increase in the amount of the GnT-V-produced branch. Mechanistically, shedding (cleavage and extracellular secretion) of GnT-V mediated by signal peptide peptidase-like 3 (SPPL3) protease is greatly inhibited by blocking maturation of cellular N -glycans, resulting in an increased level of GnT-V protein in cells. Alteration of cellular N -glycans hardly impairs expression or localizati...
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USANA Health Sciences Voted "Best Company In Network Marketing" By MLM Insider Online - PR Newswire

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SALT LAKE CITY , Feb. 4, 2013 /PRNewswire/ -- USANA Health Sciences Inc. (NYSE: USNA), a global nutritional company, has been voted 2012's "Best Company In Network Marketing" by MLM Insider Online. This is the 16 th consecutive year USANA has earned the distinguished title during its 20 years of business. (Logo:  http://photos.prnewswire.com/prnh/20120807/LA53885LOGO) "To earn this title for the 16 th time is a great honor and accomplishment," said Dan Macuga, USANA's chief communications officer. "To know our Associates support us is both gratifying and reassuring that we are doing everything we can to provide them with the information, tools and guidelines they need to succeed in the industry. We are appreciative of their continued support and enthusiasm toward the company." USANA, a longtime member of the Direct Selling Association, is also an active participant of the DSA Code of Ethics Communication Initiative, enabl...
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Flanked by a Ferrari, a Maserati, a Bentley, a Rolls-Royce and a Lamborghini, Dallin Larsen paced the stage, swigging deeply from a bottle in his hand. "I'll tell you what," said the tanned 49-year-old, opening his arms to the 4,000-person crowd, people are "looking for something they can count on, they can depend on, that's constant." The stirring scene would not be out of place at a megachurch revival—except Larsen's event, organized this June in Orlando, Fla., was bent on earning sales rather than salvation. The object of hope was not God but a dark purple fruit juice called MonaVie. The rich syrupy blend of Brazilian açai (pronounced "ah-sigh-ee") berries and 18 other fruits has gained a cult following among those who say it can kill pain, disease and malnutrition. Packaged in wine bottles like the one Larsen gripped onstage, MonaVie retails for around $40 a pop and isn't available in stores. Instead, the Utah-based company tore a pag...
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